Laura Heisler was born in the suburbs of Chicago, Illinois, and spent her childhood outside of Washington D.C. She made her film debut in the short film Wrist, released in the year 2000, and since then has appeared in a variety of films, including Coverage, Coach, Cold Souls, Forged, and YellowBrickRoad, which premiered at the Slamdance Film Festival.
As a seasoned stage actor, Laura has had the opportunity to appear in numerous world premieres Off-Broadway and regionally. Some of her notable roles include Sarah Ruhl's Eurydice, Elizabeth Meriwether's The Mistakes Madeline Made, David Adjmi's Stunning, and Mark Schultz's Everything Will Be Different. In 2007, she made her Broadway debut as Edward Ashbrook in Coram Boy, directed by Melly Still.
In 2011, Laura received nominations for both a Lucille Lortel Award and a Drama League Award for her work in Kin, written by Bathsheba Doran and directed by Sam Gold, which premiered Off-Broadway at Playwrights Horizons.